pGLO Lab

1.

Obtain your team plates.  Observe your set of  “+pGLO” plates under room light and with UV light.  Record numbers of colonies and color of colonies. Fill in the table below. Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB 20 tan/white violet - pGLO LB/amp 0 the color of nonexistence "" + pGLO LB/amp 10 tan/white violet + pGLO LB/amp/ara 6 tan/white bright glowing green

2.	What two new traits do your transformed bacteria have?
	These bacteria are now resistant to ampicillin and will glow in the presence of arabanose sugar.
3.	Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
	After two days, there were about 100 billion bacteria. Assuming the bacteria divided once and hour, a pretty typical rate, the amount of bacteria in the original 100 uL would be around 100 billion divided by 2^24 or about 6000
4.	What is the role of arabinose in the plates?
	It reacted with the pGLO to make the plate of bacteria glow a bright, florescent green.
5.	List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
	Used to label spermatoza, too help see microorganisms better, and it can be used as a reporter gene.
6.	Give an example of another application of genetic engineering. It can be used in agriculture, to make crops pick up DNA that can make them resistant to certain plagues, therefore having a positive effect on the harvest.